Salivary amylase lab report

The same was done with 1 mL of maltose solution into another two tubes. Next, pH levels were tested. This step was repeated every minute to test the presence of starch.

This was repeated every minute. The second tube changed from blue to yellow. Like most proteins, they are synthesized by the ribosomes in the cell. Results Within the indicators experiment, the first test tube changed from clear to dark purple.

Amylase is an type of enzyme. This is shown in Figure 1. Immediately a drop of each mixture was transferred to a separate well on a spot plate and a drop of I2KI was added. The activity of amylase was then observed through three reaction mixtures. Immediately a drop of the reaction was transferred to the spot plate and 1 drop of I2KI was added.

Graham, and Peter D. Into one of the test tubes of maltose and one of starch, 5 drops of I2KI were added. The tubes were observed for color change, indicating the presence of maltose. The color was observed with the passage of time to conclude whether amylase activity was present or not. In two test tubes, a 1 mL millimeter sample of starch solution was pipetted.

Finally, the effects of enzyme concentration were tested. Maltose was shown to be present in all three test tubes. All three tubes had maltose present. The Effects of Enzyme Concentration on Activity Discussion In the indicator experiment, it was necessary to test what each indicator marked.

Literature Cited Brooker, Robert J.Mr. Ulrich/Mrs. Ryan.

Regents Biology. Salivary Amylase Lab.

The Effects of Temperature, pH and Enzyme Concentration on Amylase

Background: Enzymes are protein catalysts. This means they are chains of amino acids with a particular shape that allows them to interact with a specific molecule called a substrate to bring about a chemical reaction.

Lab ReportAnnotated ab Report The fundamental purpose of this lab was to examine how enzyme concentration affects the rate at which an enzymatic reaction takes place. It was also our purpose to examine how the concentration of a substrate, a product and an enzyme can affect the direction of an enzymatic reaction.

Salivary Amylase. Lab Exercise 1: Digestion of Starch by Salivary Amylase The digestion of a carbohydrate such as starch begins in the mouth, where is it mixed with saliva containing the enzyme salivary amylase.

amylase lab report. Theresa Sterner Bio Lab 04 November The Effects Environmental Temperature and pH have on the Activity of Porcine Pancreatic Amylase Introduction There are many types of enzymes and each has a specific job.

Enzymes are particular types of proteins that help to speed up some reactions, such as reactants going to products. Salivary amylase is a powerful enzyme, and in order to study it, we will need to dilute it.

Begin by collecting 2 mL of saliva in a graduated cylinder. Use your squeeze bottle to wash Lab Report Sheet: Enzyme Activity of Salivary Amylase Part I. Testing enzyme activity in your diluted saliva.

Salivary amylase, as the name predicts, is found in saliva. This enzyme readily hydrolyzes starch. Amylase steadily removes maltose molecules from starch chains (Karp, ). This is done by hydrolyzing the a-1,4 glycosidic bonds between glucose subunits in the chain.

This is %(15).

Salivary amylase lab report
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